Hemostatic Agents in Combination with Diovine for Local Treatment of Simulated Bleeding Gastric Ulcers

Evgeniy F. Cherednikov, Alexandr A. Glukhov, Mikhail N. Romantsov, Yuri V. Maleev, Sergey V. Barannikov, Irina A. Shkurina, Anastasiya T. Vysotskaya, Evgeniy S. Ovsyannikov

International Journal of Biomedicine. 2020;10(2):138-141.
DOI: 10.21103/Article10(2)_OA10
Originally published June 15, 2020.


Background: Acute gastroduodenal bleeding (GIB) is one of the urgent and complex problems of emergency surgery. The objective of our study was to investigate the performance of powder-like hemostatic agents in combination with granular Diovine for bleeding management in experimental gastric ulcers.
Methods and Results: Experimental studies in vivo were conducted on 15 mongrel dogs, using the model of gastric ulcer by E. F. Cherednikov. During esophagogastroscopy, all animals were exposed to 2 bleeding gastric ulcers (experimental and control). Experiments were performed with 30 bleeding gastric ulcers. In experimental ulcers, endoscopic hemostasis was carried out by sufflations on a bleeding defect of powder-like Gelplastan and NovoSeven (lyophilized powder) in combination with Diovine. In control ulcers, endoscopic treatment was not performed. The experimental studies showed that in experimental ulcers, bleeding stopped at 3.9(3.5;4.9) sec (P<0.001); their hemostasis was stable without  recurrent bleeding. In control ulcers, bleeding stopped at 27.5(24.0;31.0) sec (P<0.001) and 5 episodes of recurrent bleeding were noted. The drug composition (Gelplastan, NovoSeven, and Diovine) has a hemostatic effect and accelerates healing processes of experimental bleeding ulcers. A complete healing of experimental gastric ulcers occurred in 8.0(8.0;9.0) days with a moderate scar that does not deform the stomach wall. Complete healing of control gastric ulcers occurred in 15.5(14.0;16.0) (P<0.001) days of observations.
Conclusion: The combined use of Gelplastan, NovoSeven, and Diovine for the local treatment of experimental bleeding gastric ulcers in dogs leads not only to an effective termination of hemorrhage, but also promotes reparative regeneration, synchronization of interaction of cell structures and acceleration of the healing time of simulated ulcers.

experimental gastric ulcer • gastroduodenal bleeding • endoscopic hemostasis • healing processes

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Received December 31, 2019.
Accepted March 11, 2020.
©2020 International Medical Research and Development Corporation.