7-(1-Methyl-3-Pyrrolyl-)-4,6-Dinitrobenzofuroxan Reduces the Frequency of Antibiotic Resistance Mutations Induced by Ciprofloxacin in Bacteria

ORIGINAL ARTICLE
Vladimir A. Chistyakov, PhD, ScD¹; Evgeniya V. Prazdnova, PhD¹; Evgeniya Y. Kharchenko¹; Sergey V. Kurbatov, PhD, ScD¹; Mikhail M. Batiushin, PhD, ScD²; Ekaterina S. Levitskaya, PhD²*; Maria S. Mazanko, PhD¹; Mikhail N. Churilov¹

¹Southern Federal University; ²Rostov State Medical University; Rostov-on-Don, the Russian Federation

*Corresponding author: Ekaterina S. Levitskaya, PhD. Rostov State Medical University, Rostov-on-Don, Russia.  E-mail: es.med@mail.ru

Published: September 12, 2016.  DOI: 10.21103/Article6(3)_OA15

Abstract: 

The aim of the present study was to investigate biological properties of the novel nitrobenzoxadiazole derivative 7-(1-methyl-3-pyrrolyl-)-4,6-dinitrobenzofuroxan.

Materials and Methods: We used a bioluminescent test based on a set of lux-biosensors, which are genetically modified E.coli strains able to react on different types of factors that can induce an SOS-response with light emission. The spontaneous and induced mutation frequencies of antibiotic resistance in E. coli were determined by methods of classical genetics of microorganisms.

Results: 7-(1-methyl-3-pyrrolyl-)-4,6-dinitrobenzofuroxan demonstrated inhibition of SOS-response in a biosensor model system and significantly reduced the frequency of spontaneous mutations and mutations induced by ciprofloxacin of antibiotic resistance.

Conclusion: Based on our data, we can recommend using compound-1 as a starting point for the development of drugs that block mutagenesis associated with the emergence of antibiotic-resistant bacteria.

Keywords: 
antibioitic resistance; fluoroquinolones; SOS-response; SOS-inhibitors; nitrobenzoxadiazole derivatives; antimutagens
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Int J Biomed. 2016;6(3):228-232. © 2016 International Medical Research and Development Corporation. All rights reserved.