Ion Exchange Properties of the Erythrocyte Surface Protein Band 3 and its Role in the Process of Oxygenation during CMV Infection in the Third Trimester of Pregnancy

ORIGINAL ARTICLE
Michael T. Lucenko, PhD, ScD*; Irina A. Andrievskaya, PhD, ScD; Artem G. Mironenko

Far Eastern Scientific Center of Physiology and Pathology of Respiration, Siberian Branch of the Russian Academy of Sciences; Blagoveshchensk, the Russian Federation

*Corresponding author:  Prof.  Michael  T.  Lucenko, PhD,  ScD,  Academician  of  RAS,  Head  of  Far  Eastern  Scientific Center  of  Physiology  and  Pathology  of  Respiration  SB  of  RAS, Blagoveshchensk, Russia. E-mail: Lucenkomt@mail.ru

Published: December 16, 2016.  DOI: 10.21103/Article6(4)_OA4

Abstract: 

The aim of our study was to determine the nature of the ion exchange properties of EPB3 and its participation in the process of oxygenation with CMV infection (CMVI) in the third trimester of pregnancy.
Material and Methods: The study included 105 pregnant women (the third trimester of gestation from 26 to 32 weeks): 35 CMV-seropositive pregnant women (the main group - Group 1) with CMVI exacerbation and anti-CMV IgG antibody titer of 1:1600, 35 CMV-seropositive pregnant women (the comparison group - Group 2) with latent CMVI and an anti-CMV IgG antibody titer of 1:800, and 35 CMV-seronegative pregnant women (the control group).
The study of the protein spectrum of the erythrocyte membrane was carried out by analytical separation in the presence of 0.1% SDS on a one-dimensional 7.5%-10% gradient polyacrylamide gel. The oxyhemoglobin content was determined spectrophotometrically by the method of Malloy and Evelyn (1969); the total and bound-to-hemoglobin ATP and the total and inorganic phosphorus were determined by IS Luganov and MN Blinov (1975), the activity of ouabain-sensitive Na+/K+-ATPase by Kazennova's method (1986) and the levels of  Na+ and K+ in plasma and RBCs by using "Vital Best" (Russia) sets.
Results: CMVI exacerbation in the third trimester of pregnancy is associated with a decrease in the content of EPB3 in RBC membranes due to increased proteolytic processes, which causes disturbances in its structural and functional properties. Thus, ion transport and association of the cytoplasmic domain of the protein with deoxyhemoglobin are changed and disrupt the processes of oxygenation.

Keywords: 
cytomegalovirus (CMV), erythrocyte surface protein band 3 (EPB3), pregnancy, Na+/K+-ATPase, oxyhemoglobin
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Int J Biomed. 2016;6(4):271-275. © 2016 International Medical Research and Development Corporation. All rights reserved.