Fractionating the Plant Extract in Mini Volumes to Purify the Potential Antivirals from Terminalia Chebula

Innokentii S. Solovarov, Maxim A. Khasnatinov, Tatyana A. Shishlyannikova, Natalie A. Lyapunova, Galina A. Danchinova

 
International Journal of Biomedicine. 2019;9(1):57-60.
DOI: 10.21103/Article9(1)_OA11
Originally published March 15, 2019

Abstract: 

Background: The preliminary experiments indicated that a 30% aqueous extract of seeds of the Terminalia chebula has a significant neutralizing activity against tick-borne encephalitis virus (TBEV). In this study, we developed the most gentle approach to purify the antiviral substance.
Methods and Results: The extract of T. chebula seeds was fractionated using gel filtration in Sephadex G-200 and G-50. The fractions were tested in vitro for the presence of a direct antiviral action against TBEV and profiled using liquid chromatography-mass spectrometry (LC-MS). Antiviral activity was detected in eight fractions from Sephadex G-200 and in three fractions from Sephadex G-50 gel filtration. The chromatogram of the most active Sephadex G-50 fraction exhibited three base peaks with a retention time of 3, 5 and 25.5 minutes. The MS of individual peaks revealed seven compounds with mass-to-charge ratios 114.03; 279.16; 290.91; 301.15; 579.3; 354.03, and 414.09 that were present at relatively high concentrations.
Conclusion: In spite of a small sample, it was possible to detect antiviral activity in the fractionated plant extract using convenient virological methods, as well as to separate the components of the single virus-neutralizing fraction using the LC-MS approach. The purity of resulting preparation needs to be improved.

Keywords: 
flavivirus • novel antiviral activity • chromatography • mass spectrometry
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Received December 4, 2018.
Accepted January 9, 2019.
©2019 International Medical Research and Development Corporation.