Multicomponent Polysaccharide Essential Formula of Wound Healing Medicines Enriched with Fibroblast Growth Factor

ORIGINAL ARTICLE
Ekaterina V. Silina, Nikolay V. Khokhlov, Victor A. Stupin, Natalya E. Manturova, Vitaliy I. Vasin, Evgeniy V. Velikanov, Anton L. Popov, Vadim B. Gavrilyuk, Elena B. Artyushkova, Mikhail P. Gladchenko, Alexander V. Ivanov, Victor T. Dudka, et al.

 
International Journal of Biomedicine. 2019;9(3):247-250.   
DOI: 10.21103/Article9(3)_OA12
Originally published September 15, 2019  

Abstract: 

The purpose of this research work was the creation of a safe and effective wound healing drug, in the multicomponent polysaccharide base of which the fibroblast growth factor is integrated in the optimal concentration.
Materials and Methods: The present work was carried out in three stages. In Stage 1, the essential formula of a wound healing drug was created. In order to optimize the composition of the base formula, studies were conducted with various concentrations of structure-forming polysaccharides. In Stage 2, we performed technical tests of the gel composition of the medical product on hMSCs culture, and determined the optimal concentration of the growth factor, which was added to the base formula developed at Stage 1 in different concentrations. In Stage 3, an experimental safety study of the developed gel composition, including growth factors, was performed on laboratory animals. In an experimental safety study (subchronic toxicity) on a wound model, sexually mature male rats and Wistar females of the same age (8–9 months) were used. In all animals, 2 wounds of standard size were modeled on the back. Taking into account the weight and gender, rats were distributed evenly into two experimental groups. Group 1 included rats we treated with a polysaccharide gel based on polymers. This polysaccharide gel included rhFGF-b. Group 2 was used as control (without treatment).
Results: The laboratory, histological and gravimetric studies of the internal organs and wounds of the animals in the experimental and control groups did not reveal any regular pathomorphological changes indicating the presence of the general toxicological properties of the studied products. This indicates the safety in vivo of the product being developed.
Conclusion: The studies conducted in vitro and in vivo allow us to proceed with studies determining the effectiveness of the developed wound healing medical product on laboratory animals.

Keywords: 
regeneration • wound healing • fibroblast growth factor • stem cells
References: 
  1. Sorg H, Tilkorn DJ, Hager S, Hauser J, Mirastschijski U. Skin wound healing: an update on the current knowledge and concepts. Eur Surg Res. 2017;58(1-2):81–94. doi: 10.1159/000454919.
  2. Cañedo-Dorantes L, Cañedo-Ayala M. Skin Acute Wound Healing: A Comprehensive Review. Int J Inflam. 2019;2019:3706315. doi: 10.1155/2019/3706315.
  3. Chouhan D, Dey N, Bhardwaj N, Mandal BB. Emerging and innovative approaches for wound healing and skin regeneration: Current status and advances. Biomaterials. 2019;216:119267. doi: 10.1016/j.biomaterials.2019.119267.
  4. Gaspar-Pintiliescu A, Stanciuc AM, Craciunescu O. Natural composite dressings based on collagen, gelatin and plant bioactive compounds for wound healing: A review. Int J Biol Macromol. 2019;138:854-865. doi: 10.1016/j.ijbiomac.2019.07.155.
  5. Oguntibeju OO. Medicinal plants and their effects on diabetic wound healing. Vet World. 2019;12(5):653-663. doi: 10.14202/vetworld.2019.653-663.
  6. Deptuła M, Zieliński J, Wardowska A, Pikuła M. Wound healing complications in oncological patients: perspectives for cellular therapy. Postepy Dermatol Alergol. 2019;36(2):139-146. doi: 10.5114/ada.2018.72585.
  7. Silina EV, Stupin VA, Gabitov RB. Collagen role in the mechanisms of chronic wounds healing diabetic foot syndrome. Klinicheskaya Meditsina. 2018;96(2):106-115. doi: 10.18821/0023­2149­2018­96­2­106­115
  8. Mandla S, Davenport Huyer L, Radisic M. Review: Multimodal bioactive material approaches for wound healing. APL Bioeng. 2018;2(2):021503. doi: 10.1063/1.5026773.
  9. Stupin VA, Gabitov RB, Sinelnikova TG, Silina EV. Biological mechanisms of the chronic wound ​​and diabetic foot healing: The rolle of collagen. Serbian Journal of Experimental and Clinical Research. 2018;19(4):373-382. doi: 10.2478/SJECR-2018-0077.
  10. Brooker JE, Camison LB, Bykowski MR, Hurley ET, Yerneni SS, Campbell PG, et al. Reconstruction of a Calvarial Wound Complicated by Infection: Comparing the Effects of Biopatterned Bone Morphogenetic Protein 2 and Vascular Endothelial Growth Factor. J Craniofac Surgery. 2019;30:260–264. doi: 10.1097/SCS.0000000000004779.
  11. Ho TC, Tsai SH, Yeh SI, Chen SL, Tung KY, Chien HY, et al. PEDF-derived peptide promotes tendon regeneration through its mitogenic effect on tendon stem/progenitor cells. Stem Cell Res Ther. 2019;10(1):2. doi: 10.1186/s13287-018-1110-z.
  12. Yang R, Liu F, Wang J, Chen X, Xie J, Xiong K. Epidermal stem cells in wound healing and their clinical applications. Stem Cell Res Ther. 2019;10(1):229. doi: 10.1186/s13287-019-1312-z.
  13. Park JW, Hwang SR, Yoon IS. Advanced Growth Factor Delivery Systems in Wound Management and Skin Regeneration. Molecules. 2017;22(8). pii: E1259. doi: 10.3390/molecules22081259.
  14. Marck RE, Gardien KLM, Vlig M, Breederveld RS, Middelkoop E. Growth factor quantification of platelet-rich plasma in burn patients compared to matched healthy volunteers. Int J Mol Sci. 2019; 20(2). pii: E288. doi: 10.3390/ijms20020288.

Download Article
Received August 13, 2019.
Accepted September 12, 2019.
©2019 International Medical Research and Development Corporation.