Microstructure of Temporomandibular Joint Cartilage after Intra-Articular Betamethasone Sodium Phosphate/Betamethasone Dipropionate Injection during the Early Stage of Experimental Osteoarthrosis

Irina N. Kostina, PhD*; Irina E. Valamina, PhD

Ural State Medical University; Ekaterinburg, Russian Federation.

*Corresponding author: Irina N. Kostina, PhD. Ural State Medical University, Ekaterinburg, Russian Federation. E-mail: kostinastom@yandex.ru

Published: June 22, 2014.


Objective: to study the morphological changes in cartilage after a single intra-articular betamethasone sodium phosphate (BSP)/betamethasone dipropionate (BDP) injection  during the early stage of experimental osteoarthrosis (OA) of the temporomandibular joint (TMJ).

Material and Methods: The experiment was performed on 18 male rabbits aged 6 months .The first group consisted of 9 healthy rabbits. The second group included 9 rabbits with mechanically induced TMJ OA.  For 5 days, 3 hours daily, a load (with a force of 200N) on the TMJ was imposed.  In the left TMJ of the second group of rabbits, betamethasone was injected intra-articularly in different doses: 0.1 ml (n=3), 0.3 ml (n=3), and 0.5 ml (n=3). The right TMJ was used for comparison. A combined anesthesia was applied each experimental day. Rabbits of both groups were sacrificed on days 7, 14, and 30 with introductory combined anesthesia and intravenous injection of Zoletil 100® 20 mg/kg to stop their breathing.

Results: Betamethasone caused destruction of the chondrocytes, fragmentation of collagen fibers, deficit of proteoglycans (PGs) and glycosaminoglycans (GAGs), thinning of the cartilage, and contributed to the progression of TMJ OA.

Conclusion: The optimal dose of BSP/BDP for intra-articular injection in the early stages of TMJ OA must be within the range of 0.1-0.3 ml|0.7-1.5 mg.

temporomandibular joint cartilage; experimental osteoarthrosis; betamethasone sodium phosphate/betamethasone dipropionate.

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Int J Biomed. 2014; 4(2):99-103. © 2014 International Medical Research and Development Corporation. All rights reserved.