Diphenyl Derivatives: Cytotoxicity, Antiviral and IFN-inducing Activities in vitro

Olena S. Bogorad-Kobelska¹*, Nadiya M. Zholobak, PhD¹, Olena V. Dolga, PhD², Georgiy V. Maltzev³, Sergey A. Lyakhov, PhD³, Sergey A. Andronati, PhD, ScD³, Mykola Ya. Spivak PhD, ScD¹

¹D. K. Zabolotny Institute of Microbiology and Virology of NAS, Kyiv, Ukraine

²O. O. Bogomoletz Institute of Physiology of NAS, Kyiv, Ukraine

³O. V. Bogatsky Physico-Chemical Institute of NAS, Odessa, Ukraine

*Corresponding author: Olena S. Bogorad-Kobelska, Department of Interferon and Immunomodulators, D. K. Zabolotny Institute of Microbiology and Virology of National Academy of Sciences. 154, Zabolotny str. 03680, Kyiv, Ukraine. Tel/Fax: 380-44-5269425.E-mail: alenask-13@rambler.ru


Background: the search for new antiviral drugs is the pressing task ahead of scientists all over the world. Ideally, the new drugs should be less cytotoxic and act directly on the virus or induce the production of body’s own interferon. Diphenyl derivatives are analogues of tilorone (known in the Ukraine, such as «Amixine IC») with less cytotoxicity and higher interferon-inducing activity. In this study, the toxicity, antiviral and interferon-inducing activities of diphenyl derivatives have been compared for the different cell cultures.

Methods: Cytotoxicity, IFN-inducing and antiviral activity of diphenyl derivatives were studied using cell lines L929, PST and Vero.

Results: Diphenyl derivatives were found to be less toxic when compared with tilorone. Also, these compounds induced interferon production on L929 and PST cell lines and gave 50% antiviral protection on the Vero cell line which cannot produce interferon.

Conclusions: The compounds tested are capable of protecting cells against viral cytopathic effect, not only through interferon production, but also through some other mechanisms of antiviral activity. Further study of these mechanisms appears to be promising.

antiviral drugs; interferon induction; diphenyl derivatives; tilorone.
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Int J Biomed. 2011; 1(3): 153-157. © 2011 International Medical Research and Development Corporation. All rights reserved.