Influence of Oxidative Damage on Lithogenesis in Experimental Nephrolithiasis

Valeriy M. Bryukhanov, PhD, ScD, Yury G. Motin, PhD*, Alexander Y. Zharikov, PhD, Jakov F. Zverev, PhD, ScD, Vyacheslav V. Lampatov, PhD, ScD, Natalya V. Motina

Altai State Medical University, Barnaul, Russian Federation

*Corresponding author: Yury G. Motin, PhD, Assistant Professor, Department of Histology, Altai State Medical University, 40, Lenin ave., 656038, Barnaul, Altai Krai, Russian Federation. Tel: 7-3852-260864 (work), 7-913-2139009 (mobile).


To assess the impact of oxidative damage to the kidney structure and the severity of lithogenesis, a morphological study of rat kidneys was conducted with experimental oxalate nephrolithiasis. Structural changes were assessed in the kidney medulla, particularly the distribution and size of the calcium compounds. Using immunohistochemistry, the expression of the severity indices of oxidative damage (malondialdehyde) and antioxidant defenses (mitochondrial superoxide dismutase) were determined. On the model of experimental oxalate nephrolithiasis in the rat kidney at the level of light-optical reconstruction, signs of histopathological alterations were revealed in the organ, along with the presence of calcium compounds in the kidney tubular system and interstitial cells. Also, the morphological symptoms of activation of the oxidative damage to the tissues and cells, reducing the antioxidant protective mechanisms of the enzymes were noted. Usage of α-tocopherol revealed a definite reduction in the severity, in terms of the structural adjustment to renal oxidative damage of the tissues and cells, and preservation of the activity of the antioxidant defense system, as well as a reduction in the number and size of the calcium deposits formed. 

experimental oxalate nephrolithiasis; morphology of kidney; free radical oxidation; antioxidants.

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Int J Biomed. 2011; 1(4):228-230. © 2011 International Medical Research and Development Corporation. All rights reserved.