Interleukin-1B and Interleukin-1 Receptor Antagonist in Patients with Helicobacter pylori Associated Diseases

Olga V. Shtygasheva, PhD, ScD, Elizaveta S. Ageeva, PhD*.

Nicolay F. Katanov State University of Khakassia Abakan, Russian Federation

*Corresponding author: Elizaveta S. Ageeva, PhD, Head of Fundamental Medicine and Hygiene Department, Federal State Budgetary Educational Institution of Higher Professional Education “Katanov State University of Khakassia”, 178, Pushkin str., Abakan, 655004, Khakassia, Russian Federation. Tel: 7-3902-342720,


The ethnic people of the Republic of Khakassia (the Khakas) with ulcer disease show a significant T-cell activation and humoral immune response when compared with the Europoids. The reasons for such differences could be due to certain ethno -specific allelic variants of the interleukins, which considerably change the degree of cytokine expression. The aim was to study the peculiarities of the association of the interleukin-1 (IL-1) gene polymorphisms and interleukin-1 receptor antagonist (IL-1Ra). Patients with chronic gastritis and ulcer disease were examined using the restriction analysis method. The most wide-spread allelic variants among the Khakas were discovered to be CC IL-1β and R4R4 IL-1Ra. In this study, we suggest the necessity to define the population`s risk and the protective genotypes that promote Helicobacter pylori-associated ulcer disease among the Khakas people.

Helicobacter pylori; ulcer disease; the Khakas; Th1/Th2 immune response; gene polymorphism; interleukin-1.

1. Perez-Perez GI, Rothenbacher D, Brenner H. Epidemiology of Hlicobacter pylori Infection. Helicobacter 2004; 9:1-6.
2. Yamaoka Y, Malaty HM, Osato MS. Concervation of Helicobacter pylori genotypes in different ethnic groups in Houston, Texas. J Infect Dis 2000; 181(6):2083–2086.
3. Tsukanov VV, Barkalov SV, Tonkikh YuA, Shtygasheva OV, et al. The incidence rate of CagA-strains of Helicobacter pylori and ulcer disease among the population of Eastern Siberia. Therapeutic Archive 2007; 2:15-18.[in Russian].
4. Shtygasheva OV, Tsukanov VV. The association of саgA and vacA Helicobacter pylori strains and ulcer disease in the organized population of Abakan. The Russian Journal of Gastroenterology, Hepatology and Coloproctology 2004; 2:84-87. [in Russian].
5. Kononov АV. Molecular genetics and inflammatory phenotype caused by Helicobacter pylori. Omsk Scientific Journal 2007; 3(61):17-23. [in Russian].
6. Wolfe MM. Hypergastrinemia and colonic neoplasia: coincidental or related? Gastroenterology 1992; 103:1361–1363.
7. Liou JM, Lin JT, Chang CY, Chen MJ, et al. Levofloxacin-based and clarithromycin-based triple therapies as first-line and second-line treatments for Helicobacter pylori infection: a randomised comparative trial with crossover design. Gut 2010; 59:572-578.
8. Gromova AYu, Simbirtsev A. The polymorphism of IL-1 gene family. Cytokines and inflammation 2005; 2:24-35. [in Russian].
9. Wilkinson RJ, Patel P, Llewelyn M, et al. Influence of polymorphism in the genes for the interleukin (IL)–1 receptor antagonist and IL-1β on tuberculosis. J Exp Med 1999; 189:1863-1873.
10. Landi S, Moreno V, Gioia-Patricola L, et al. Association of common polymorphisms in inflammatory genes interleukin (IL)6, IL8, tumor necrosis factor- , NF- B1, and peroxisome proliferator-activated receptor   with colorectal cancer. Cancer Res 2003; 63:3560–3566.
11. McCarron SL, Edwards S, Evans PR, et al. Influence of cytokine gene polymorphisms on the development of prostate cancer. Cancer Res 2002; 62:3369–3372.
12. Laine ML, Farre MA, Bart J, et al. The Mouthwash: A Non-Invasive Sampling Method to Study Cytokine Gene Polymorphisms. Periodontol 2000;71(8):1315-1318.
13. Joos L, McIntyre L, Ruan J, et al. Association of IL-1B and IL-1 receptor antagonist haplotypes with rate of decline in lung function in smokers. Thorax 2001; 56:863-866.
14. Tarlow JK, Blakemore AI, Lennard A, et al. Polymorphism in human IL-1 receptor antagonist gene intron 2 is caused by variable numbers of an 86-bp tandem repeat. Hum Genet 1993; 91:403-404.
15. Shtygasheva O.V. Clinicopathologic characteristics of ulcer disease among the population of the Republic of Khakassia. ScD thesis. Krasnoyarsk, 2005. [in Russian].
16. Lee SG, Kim B, Choi W, et al. Lack of association between pro-inflammatory genotypes of the interleukin-1 (IL-1B -31 C/+ and IL-1RN *2/*2) and gastric cancer/duodenal ulcer in Korean population. Cytokine 2003; 21:167–71.
17. Palli D, Saieva C, Luzzi I, et al. Interleukin-1 gene polymorphisms and gastric cancer risk in a high-risk Italian population. Am J Gastroenterol 2005; 100:1941–1948.
18. Bidwell J, Keen J, Gallagher G, et al. Cytokine gene polymorphism in human disease: on-line database. Genes and Immun 1999; 1:3–19.
19. Witkin SS, Gerber S, Ledger WJ. Influence of interleukin-1 receptor antagonist gene polymorfim on disease. Clin Infect Dis 2002; 34(2):204–209.
20. Chen A, Li CN, Hsu PI, et al. Risks of interleukin-1 genetic polymorphisms and Helicobacter pylori infection in the development of gastric cancer. Aliment Pharmacol Ther 2004; 20:203–211.
21. Goodwin CD. Duodenal ulcer, Campylobacter Pylori and the «leaking roof» concept. Lancet 1988; 2(8626/8627):1467–1469.
22. Kononov АV. Genetic regulation and inflammatory phenotype in the case of Helicobacter pylori-infection. Pathology Archive 2009; 71(5):57-63. [in Russian].
23. Shabaldin AV, Ostaptseva AV, Akhmatyanova VR, et al. Polymorphism of interleukin-1 receptor antagonist in the non-ethnic and ethnic people of Kemerovo Region. Immunology 2007; 2:72-74. [in Russian].

The fully formatted PDF version is available.

Download Article

Int J Biomed. 2012;2(2):117-123.©2012 International Medical Research and Development Corporation. All rights reserved.