The NOS3 T-786C (rs2070744) Gene Polymorphism in Patients of Uzbek Nationality with Chronic Heart Failure

CLINICAL RESEARCH
Ch.A. Abdullayeva¹ ; Kh.Ya. Karimov, PhD, ScD²; U.K. Kamilova³, PhD, ScD*; K.T. Boboev, PhD²; N.B. Nuritdinova¹.

¹Tashkent Medical Academy; ²Research Institute for Hematology and Blood Transfusion; ³The Republican Specialized Scientific-Practical Medical Centre of Therapy and Medical Rehabilitation; Tashkent, Uzbekistan

*Corresponding author: Umida K. Kamilova, PhD, ScD. The Republican Specialized Scientific - Practical Medical Centre of Therapy and Medical Rehabilitation; Tashkent, Uzbekistan. E-mail: umida_kamilova@mail.ru

Abstract: 

This study aimed to evaluate the role of endothelial nitric oxide synthase (eNOS, type 3, NOS3) T-786C (rs2070744) gene polymorphism in chronic heart failure (CHF) manifestation in Uzbek patients. The study involved 81 CHF male patients, Uzbeks, aged from 41 to 70 years. The control group comprised 75 healthy, age-matched, randomly selected male persons. Genomic DNA was isolated and SNP genotyping was performed by using a polymerase chain reaction with specific primers followed by restriction fragment length polymorphism analysis. In CHF patients, the observed frequency of genotypes was as follows: TT = 0.556; CT = 0.432; CC = 0.012; the expected frequency of genotypes was as follows: TT = 0.595; CT = 0.352; CC = 0.052 (χ2=4.14, P=0.04). Deviation from Hardy–Weinberg equilibrium was noted due to an excess of heterozygosity. The results of our study have shown a significant association between CT genotype of NOS-3 T-786C gene polymorphism and CHF manifestation in patients of Uzbek nationality.

Keywords: 
endothelial nitric oxide synthase; T-786C (rs2070744) gene polymorphism; chronic heart failure.
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IJBM 2014; 4(4) Suppl 1:S12-S14. © 2014 International Medical Research and Development Corporation. All rights reserved.