HLA Typing and Chromosomal Aberrations Caused by Chemical Agents

Gulnara S. Agsamova, PhD

Tashkent Medical Academy; Tashkent, Uzbekistan

*Corresponding author: Gulnara S. Agzamova, PhD. Head of Department of Occupational Diseases of Tashkent Medical Academy; Tashkent, Uzbekistan. E-mail: agzamova_gulya@mail.ru


The aim of this research was to perform HLA typing and determine the chromosomal aberrations in patients with chronic lead and pesticide (organophosphates and organochlorines) toxicity.

Materials and Methods: Cytogenetic studies were performed on 105 study participants. Eighty-five patients with chronic professional toxicity were divided into two groups. Group 1 consisted of 40 patients with chronic lead toxicity; Group 2 consisted of 65 patients with chronic pesticide toxicity. Cytogenetic effects were estimated using a metaphase plates technique. Cytostatic effect of studied chemicals was evaluated using PHA stimulation index.  Distribution of HLAantigens was studded in 30 patients with chronic viral hepatitis and 60 patients with work-related chronic liver disease.

Results: The frequency of chromosomal aberrations in peripheral blood lymphocytes was increased in persons with occupational exposure to pesticides. In 66.7% of workers exposed to lead, the response to PHA stimulation was very low. Our results showed that HLA- A2, A25, B16, B8, B22 antigens were determined statistically more frequently in patients with chronic liver toxicity; HLA-A2/B8, A2/B15, A25/B22 haplotypes increased the risk of liver toxicity.

chronic lead and pesticide toxicity; chromosomal aberrations; HLA antigens
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IJBM 2014; 4(4) Suppl 1:S20-S22. © 2014 International Medical Research and Development Corporation. All rights reserved.