Comparative Evaluation of Isolated and Complex Use of Hexetidine and Photoditazine in Combination with Ultrasound Therapy in the Treatment of Purulent Wounds under Experimental Conditions

ORIGINAL ARTICLE
Ivan S. Dragovoz, Dmitry S. Zotov, Maria A. Zatolokina, Arsen Yu. Grigoryan, Ekaterina S. Mishina, Vadim V. Tsymbalyuk

 
International Journal of Biomedicine. 2022;12(1):138-141.
DOI: 10.21103/Article12(1)_OA15
Originally published March 10, 2022

Abstract: 

Background: Currently, despite the significant success of the pharmaceutical industry in producing drugs with antibacterial activity, the treatment of soft tissue diseases accompanied by purulent-inflammatory processes within them remains one of the topical problems of general surgery. Not only the widespread introduction of antibacterial agents in practical medicine, but also the steady increase in the resistance of microorganisms to existing drugs leads to the need to search for new combinations aimed at the local treatment of purulent wounds.  The purpose of our work was to study the course of the wound process in a purulent wound by using hexetidine and photoditazine in combination with ultrasound treatment.
Methods and Results: This experimental study was carried out on 144 male Wistar rats. The course of the wound process was evaluated on a model of a purulent wound. The animals were divided into 4 equal groups (36 animals in each group), depending on their treatment. Treatment was carried out using daily dressings for 15 days. The dynamics of changes in the area of the wound were assessed by the planimetry method. The percentage of area reduction of the wound was calculated. A histological examination of a skin area (1.0 cm×1.5 cm) taken in the zone of a simulated purulent wound was carried out on Days 5 and 10 of the experiment. our study confirmed the effectiveness of the use of hexetidine, photoditazine, and a combination of them, along with ultrasound treatment, in the local treatment of a purulent wound. At the same time, the combined effect of an antiseptic and a photosensitizer showed significantly better results in the first and second phases of the course of the wound process than did their isolated use.

Keywords: 
purulent wound • hexetidine • photoditazine • ultrasound therapy • methylcellulose
References: 
  1. Arkhipov DV, Andreev AA, Atyakshin DA, Glukhov AA, Ostroushko AP. [Jet oxygen sorption treatment in the local treatment of purulent wounds of soft tissues]. Bulletin of Experimental and Clinical Surgery. 2020;13(1):41–45. doi:10.18499/2070-478X-2020-13-1-41-45. [Article in Russian].
  2. Katorkin SE, Bystrov SA, Lisin OE, Rozanova AA, Bezborodov AI. [Evaluation of the effectiveness of the use of modern dressings in the complex treatment of purulent wounds]. Stationary-Replacing Technologies: Ambulatory Surgery. 2019;1-2:146–152. doi:10.21518/1995-1477-2019-1-2-146-152. [Article in Russian].
  3. Bezhin AI, Pankrusheva TA, Zatolokina MA, Grigoryan AYu, Zhilyaeva LV, Kobzareva EV, Mishina ES. [Morphological features of wound surface healing when using new preparations based on carboxymethyl cellulose]. Modern Problems of Science and Education. 2015;3. Available from: https://science-education.ru/ru/article/view?id=20333. [Article in Russian].
  4. Hoffmann JP, Friedman JK, Wang Y, McLachlan JB, Sammarco MC, Morici LA, Roy CJ. In situ Treatment With Novel Microbiocide Inhibits Methicillin Resistant Staphylococcus aureus in a Murine Wound Infection Model. Front Microbiol. 2020 Jan 23;10:3106. doi: 10.3389/fmicb.2019.03106. 
  5. Duvansky VA, Eliseenko VI, Shin EF. Influence of photodynamic therapy on the reparative processes of purulent wounds. Proceedings of the 3rd International Congress "Wounds and wound infections." Moscow;2016:99–100.
  6. Belyaeva OA, Luneva AG, Kryzhevsky VV, Karol IV, Zavadetskaya EP, Oleinik EA, Krivenko EA. [Cytological monitoring of the healing of purulent wounds during their treatment using application sorption and NO-therapy. Laboratory diagnostics]. Eastern Europe. 2018;7(3):374–383. [Article in Russian].
  7. Muñoz-Sepeda PA, Blatun LA, Paskhalova YuS. The effectiveness of ultrasonic cavitation in the treatment of purulent-necrotic complications of the diabetic foot syndrome. Proceedings of the 4th International Scientific and Practical Congress "Wounds and wound infections." Moscow;2018:123–124.
  8. Conner-Kerr Т, Oesterle МЕ. Current perspectives on therapeutic ultrasound in the management of chronic wounds: a review of evidence. Chronic Wound Care Management and Research. 2017;4:89-98.

Download Article
Received February 3, 2022.
Accepted March 9, 2022.
©2022 International Medical Research and Development Corporation.