Analysis of the Association of 5-Hydroxytryptamine Receptor 2A Gene Variants in Nicotine Addiction in the Yakut Population

ORIGINAL ARTICLE
Nadezhda I. Pavlova, Aleksey A. Bochurov, Vladislav A. Alekseev, Aleksandra T. Diakonova, Vladimir V. Dodokhov, Khariton A. Kurtanov

 
International Journal of Biomedicine. 2022;12(1):151-154.
DOI: 10.21103/Article12(1)_OA18
Originally published March 10, 2022

Abstract: 

The aim of this study was to investigate the relationship between the HTR2A 1438A/G (rs6311) SNP and the risk of nicotine addiction in Yakuts.
Methods  and Results: A total of 292 people of Yakut nationality were tested (77 women and 215 men). Two groups of examined persons were formed: smokers (n=141) and non-smokers (NS, n=151). To determine the association of the studied SNP with the degree of smoking, the smoker group was divided into two subgroups: Heavy smokers (HS) and Light smokers (LS). Subjects who were smokers were classified based on their consumption of cigarettes per day (cpd), as follows: LS (n=10), those who consumed between 1 and 10 cpd, and HS (n=131), those who consumed ≥20 cpd. The study of the HTR2A 1438A/G (rs6311) SNP was performed by PCR and RFLP analysis. The A allele of the HTR2A 1438A/G (rs6311) SNP was associated with more of a risk for the HS group than the LS. Using the A allele as a risk factor, RR analysis showed a significant association with a risk factor when comparing HS with LS (RR=1.086; 95% CI=1.032-1.142; P=0.049). The analysis of the OR and RR between the HS and LS showed that the AA and AG genotypes were associated with an increased risk for heavy smoking (OR=9.714; 95% CI=1.196-78.874; RR =1.126; 95% CI=1.027-1.235; P=0.026). We cannot dismiss the possibility that our results may reflect the small sample size. Despite this potential limitation, the significant associations should not be disregarded and merit further investigation for clarification of these results.

Keywords: 
HTR2A • rs6311• SNP • addictive behavior
References: 
  1. World Health Organization. 01.17.2022. Age-standardized estimates of current tobacco use, tobacco smoking and cigarette smoking. Data by country. Available from: https://apps.who.int/gho/data/node.main.TOBAGESTDCURR?lang=en
  2. Federal State Statistics Service. Selective observation of behavioral factors affecting the health status of the population 2018. Available from: https://burstat.gks.ru/pfczn. [In Russian].
  3. Ramos Neto ES, Mágulas JO, Sousa JJ, Moura AC, Pinto GR, Yoshioka FK, Canalle R, Motta FJ. Study of polymorphic variants of the serotonin 2A receptor gene (5-HT2A) and its possible effects on smoking habits of a population from northeastern Brazil. Genet Mol Res. 2014 Oct 20;13(4):8268-77. doi: 10.4238/2014.October.20.3.
  4. Pérez-Rubio G, Ramírez-Venegas A, Noé Díaz V, García Gómez L, Elvira Fabián K, García Carmona S, López-Flores LA, Ambrocio-Ortiz E, Contreras Romero R, Alcantar-Ayala N, Sansores RH, Falfán-Valencia R. Polymorphisms in HTR2A and DRD4 Predispose to Smoking and Smoking Quantity. PLoS One. 2017 Jan 19;12(1):e0170019. doi: 10.1371/journal.pone.0170019. 
  5. Ramos Neto ES, Mágulas JO, Sousa JJ, Moura AC, Pinto GR, Yoshioka FK, Canalle R, Motta FJ. Study of polymorphic variants of the serotonin 2A receptor gene (5-HT2A) and its possible effects on smoking habits of a population from northeastern Brazil. Genet Mol Res. 2014 Oct 20;13(4):8268-77. doi: 10.4238/2014.October.20.3. 
  6. Polina ER, Contini V, Hutz MH, Bau CH. The serotonin 2A receptor gene in alcohol dependence and tobacco smoking. Drug Alcohol Depend. 2009 Apr 1;101(1-2):128-31. doi: 10.1016/j.drugalcdep.2008.11.001. 
  7. Diakonova AT, Pavlova NI, Solovyeva NA, Varlamova MA, Alexandrova TN, Kurtanov KhA. Molecular-genetic analysis of the connection of the SLC6A3 gene with nicotine addiction in Yakutia. Yakut Medical Journal. 2018;4(64):6-8. doi: 10.25789/YMJ.2018.64.01. [Article in Russian].
  8. Levran O, O'Hara K, Peles E, Li D, Barral S, Ray B, Borg L, Ott J, Adelson M, Kreek MJ. ABCB1 (MDR1) genetic variants are associated with methadone doses required for effective treatment of heroin dependence. Hum Mol Genet. 2008 Jul 15;17(14):2219-27. doi: 10.1093/hmg/ddn122. 
  9. Hsin CH, Stoffel MS, Gazzaz M, Schaeffeler E, Schwab M, Fuhr U, Taubert M. Combinations of common SNPs of the transporter gene ABCB1 influence apparent bioavailability, but not renal elimination of oral digoxin. Sci Rep. 2020 Jul 27;10(1):12457. doi: 10.1038/s41598-020-69326-y. 
  10. Pavlova NI, Diakonova AT, Alekseev VA, Borisova YP, Dodokhov VV, Dmitrieva TI, Kurtanov KhA. C3435T Polymorphism of the ABCB1 Gene in the Yakut Population. International Journal of Biomedicine 11(3) (2021) 367-371. doi: 10.21103/Article11(3) OA15.
  11. Verde Z, Santiago C, Chicharro LM, Bandrés F, Gómez-Gallego F, Rodríguez González-Moro JM, de Lucas P. Association of HTR2A-1438G/A Genetic Polymorphism With Smoking and Chronic Obstructive Pulmonary Disease. Arch Bronconeumol (Engl Ed). 2019 Mar;55(3):128-133. doi: 10.1016/j.arbres.2018.07.021.

Download Article
Received January 21, 2022.
Accepted February 28, 2022.
©2022 International Medical Research and Development Corporation.