International Journal of Biomedicine. 2023;13(1):69-72.
Originally published March 3, 2023
Currently, cervical cancer (CC) is one of the most common oncological diseases. In this regard, it is necessary to develop new research methods for a more detailed study of the occurrence and development of the disease at the molecular and cellular levels, as well as to improve the effectiveness of treatment and form a deeper understanding of the causes of relapses. The aim of this work was to study nanoparticles localized on the erythrocyte membrane—presumably HPV 16, 18, before and after radiation therapy, in patients with cervical cancer (CC).
To study the surface of red blood cells by the SEM method, venous blood samples from 17 patients with a confirmed diagnosis of CC were prepared in thin layers evenly applied to a dry, fat-free glass slide, which was dried at room temperature. To detect nanoparticles on the surface of erythrocytes by the immunofluorescence analysis, we developed a special protocol for preparing erythrocyte masses from patients diagnosed with CC. As a result of using a new method of sample preparation for immunofluorescence assay and using SEM, the hypothesis of the viral nature of nanoparticles localized on the surface of the blood erythrocytes of patients with CC was confirmed: Particles of HPV 16 and 18 are located on the cytoplasmic membrane of erythrocytes. Studies suggest that viruses attach to the erythrocyte membrane, which seems to influence the development of CC, its recurrence, and metastasis.
1. MISKAWAAN. Global Cancer Facts and Figures 2021. Available at: https://miskawaanhealth.com/cancer/global-cancer-statistics/
2. WHO. Cancer Today. Available at: https://gco.iarc.fr/today/home
3. Lushnikova PA, Sukhikh ES, Izhevsky PV, Sutygina YN, Tatarchenko MA, Pyzhova IB. [Modern Techniques for Cervical Cancer Radiotherapy]. Creative surgery and oncology. 2021;11:58-67. doi: 10.24060/2076-3093-2021-11-1-58-67. [Article in Russian].
4. Tazhibayeva KN, Sadykova AD, Tasboltayeva DT, Ormanov AN, Kaldygozova GE. Ways to improve the diagnosis and detection of cervical cancer and recurrence risk. Oncology and radiology of Kazakhstan, 2021; №4 (62): 24-27. doi: 10.52532/2663-4864-2021-4-62-24-27
5. Shakirova EZ, Zidikhanov DI. [Salvage hysterectomy after radiotherapy for cervical cancer: A literature review]. Tumors of female reproductive system. 2021;17(3):121–7. doi: 10.17650/ 1994-4098-2021-17-3-121-127. [Article in Russian].
6. Kreinina YM, Shevchenko LN, Kaskulova MK, Dykina AV, Smyslov AY, Trotsenko SD, et al. [Actual technologies of conformal radiation therapy in modern programs of treatment of relapses of cervical cancer, uterine body and ovaries]. Oncogynecology. 2020;2(34): 60-70. doi: 10.52313/22278710-2020-2-60. [Article in Russian].
7. Mansurova GB, Saidova KA, Razakov AR, Talybova SA, Agzamov OA, Chen et al. Analysis of factors influencing the recurrence of cervical cancer. Clinical and Experimental Oncology. 2018;1(3):15-18.
8. Shumeikina AO, Vavilov KV, Samoilova EA, Mansurova AS, Ponomarenko AG. The possibilities of using stereotactic radiation therapy for the treatment of recurrent cervical cancer. Materials of the VIII St. Petersburg International Cancer Forum "White Nights 2022." Tumors of the female reproductive system: radiation therapy. Issues of Oncology. 2022;3(68):231-232.
9. Mamaeva SN, Kononova IV, Ruzhansky M, Nikiforov PV, Nikolaeva NA, Pavlov AN et al. The use of scanning electron microscopy and atomic force microscopy to study the formation of nanoparticles on the surface of erythrocytes in patients with cervical cancer. International Journal of Biomedicine. 2020; 10(1):70-75. doi: 10.21103/Article 10(1)_OA12
10. Mamaeva SN, Kononova IV, Nikolaevа NA, Pavlov AN, Semenova MN, Maksimov GV. Determination of Blood Parameters using Scanning Electron Microscopy as a Prototype Model for Evaluating the Effectiveness of Radiation Therapy for Cervical Cancer. International Journal of Biomedicine.. 2021;11(1):32-38. doi: 10.21103/Article11(1)_OA6
11. Tamkovich SN, Yunusova NV, Tugutova E, Somov AK, Proskura KV, Kolomiets LA, Stakheeva MN, Grigor’eva AE, Laktionov PP, Kondakova IV. Protease Cargo in Circulating Exosomes of Breast Cancer and Ovarian Cancer Patients. Asian Pac J Cancer Prev. 2019 Jan 25;20(1):255-262. doi: 10.31557/APJCP.2019.20.1.255.
12. Tamkovich SN, Bakakina YS, Tutanov OS, Somov AK, Kirushina NA, Dubovskaya LV, et al. Proteomic analysis of circulating exosomes in healthcare and breast cancer. Russian Journal of Bioorganic Chemistry. 2017:43(2):126-134. doi: 10.1134/S1068162017020157
13. Tamkovich S, Tutanov O, Efimenko A, Grigor'eva A, Ryabchikova E, Kirushina N, Vlassov V, Tkachuk V, Laktionov P. Blood Circulating Exosomes Contain Distinguishable Fractions of Free and Cell-Surface-Associated Vesicles. Curr Mol Med. 2019;19(4):273-285. doi: 10.2174/1566524019666190314120532.
14. Zhang W, Ou X, Wu X. Proteomics profiling of plasma exosomes in epithelial ovarian cancer: A potential role in the coagulation cascade, diagnosis and prognosis. Int J Oncol. 2019 May;54(5):1719-1733. doi: 10.3892/ijo.2019.4742.
15. Islam MS, Chakraborty B, Panda CK. Human papilloma virus (HPV) profiles in breast cancer: future management. Ann Transl Med. 2020 May;8(10):650. doi: 10.21037/atm-19-2756.
Received December 12, 2022.
Accepted February 19, 2022.
©2023 International Medical Research and Development Corporation.