Effect of Cytochrome P450 2C19 681G>A Polymorphism on Premature Coronary Heart Disease

Zhong-Hai Chi, Yuan-Yuan Li, Juan Li, Zheng Zhang*, Li Jia

The Affiliated Hospital of Medical College Qingdao University, Qingdao, China

*Corresponding author: Zheng Zhang, Graduate student from Medical College Qingdao University; Qingdao, China. E-mail: 163zhangz@163.com

Published: June 25, 2015. DOI: 10.21103/Article5(2)_CR1


Objective: The aim of our study was to evaluate the association between cytochrome P450 2C19 (CYP2C19) 681G>A polymorphisms and the age of development of coronary heart disease. Additionally, the study might find some biological indicators at the gene level that could help to predict and evaluate the risk of coronary heart disease in time.  

Methods: This study included 352 individuals with coronary heart disease. Polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) was used to identify CYP2C19 681G>A. The objects were divided into wild type (GG or homozygous CYP2C19*1 wild-type) and mutant type (GA/AA or the mutant CYP2C19*2 allele) on the basis of genotype. The association between CYP2C19 gene polymorphism and the age of onset of coronary heart disease was assessed by multivariate linear regression analysis. 

Results: There was a significant association in the age of onset between the two groups (t=3.398, P=0.001), which was 58.51±12.72 years in the wild type and 53.95±11.63 years in the mutant group. The frequency of CYP2C19 681A (CYP2C19*2 allele) was 0.32, 0.268, 0.227 in different age groups, which was significantly different (χ2=10.745, P=0.005) in different groups, as well as in the genotype. The result, assessed by multiple linear regression, showed that genotype, smoking, obesity, and hyperlipidemia affect the age of onset of coronary heart disease (β was -0.167, 0.156, 0.155, and 0.112, P<0.05),  and mutually affect the age of onset of coronary heart disease. 

Conclusion: The study suggests that CYP2C19 681G>A  gene polymorphism may be one of the risk factors in susceptibility to early onset of coronary heart disease, but not an independent factor because other factors may play a synergistic role.

CYP2C19 681G>A polymorphism; premature coronary heart disease; age of onset.
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