Cytokine Gene Polymorphisms in Chronic Adenoiditis

ORIGINAL ARTICLE
Natalia V. Terskova, Natalia A. Shnayder, Andrey S. Simbirtsev, Sergey G. Vakhrushev, Dinara R. Sidorenko, Natalia V. Platonova

 
International Journal of Biomedicine. 2018;8(3):213-216.   
DOI: 10.21103/Article8(3)_OA8             
Originally published September 15, 2018  

Abstract: 

The aim of our research was to study the multiphase response in a system of pro-inflammatory and anti-inflammatory cytokines due to the additive contribution of homozygous and heterozygous genotypes for the polymorphic allelic variants of the interleukin-1β (IL-1β) and interleukin-4 (IL-4) genes in patients with chronic adenoiditis (CA).
Materials and Methods: The study included 388 children with CA. Associations between the IL1B gene (rs1143634) (C+3954T) SNP and the IL-4 gene (rs2243250) (C-589T) SNP and the clinical manifestations and clinical outcome of CA were investigated. Genotyping for the studied SNPs was performed using real-time PCR. The study of genotype-associated cytokine production in accordance with the level of concentration of IL-1β, IL-4 in blood serum with the method of solidphase EIA using horseradish peroxidase as an indicating enzyme was carried out.
Results: The presence of homozygous or heterozygous genotypes of the studied SNPs of the IL-1β and IL-4 genes was characterized with genetically determined cytokine-production forming the phenotypical polymorphism. The conducted research into congenital immunity factors with an assessment of genetically determined cytokine production has revealed 5 options of the cytokine response and their corresponding frequencies. We extrapolated the results on clinical and functional outcomes of chronic adenoiditis, which allowed us to identify non-randomness in the nature of chronic adenoiditis as a multifactorial disease.
Conclusion: The obtained data are evidence of the phenotypic-genetic heterogeneity of CA.

Keywords: 
chronic adenoiditis • interleukin-1β • interleukin-4 single nucleotide polymorphism
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Received April 10, 2018.
Accepted July 16, 2018.
©2018 International Medical Research and Development Corporation.