Polyunsaturated Fatty Acids of Blood Serum and the Assessment of their Ratios in Clinically Healthy Adults Living in the Arctic Territories of Russia

ORIGINAL ARTICLE
Fatima A. Bichkaeva, David S. Galstyan, Natalia I. Volkova, Olga S. Vlasova, Ekaterina V. Nesterova, Artyom A. Bichkaev, Boris A. Shengof, Andrey I. Popov, Nina F. Baranova

 
International Journal of Biomedicine. 2019;9(4):338-344.
DOI: 10.21103/Article9(4)_OA13
Originally published December 15, 2019

Abstract: 

Background: The study of the content of polyunsaturated fatty acids (PUFAs) in permanent residents of the Arctic territories, characterized by high activity of lipid metabolism, is of undoubted interest. The aim of this study was to assess the composition of omega-3 and omega-6 PUFAs in blood serum by gas-liquid chromatography and individual PUFA ratios in clinically healthy adults living in the Russian Arctic and Sub-Arctic regions.
Materials and Methods: A total of 1,556 healthy adult residents (the age groups of 22-35, 36-45 and 46-60 years) of the northern territories were examined. Of these, 661 people were living in the Sub-Arctic region (SAR) and 895 people in the Arctic region (AR). Analysis of PUFA composition in blood serum was  determined by gas-liquid chromatography  after transesterification to volatile fatty acid methyl esters (FAME). We determined the content of ω-3 PUIFAs: α-linolenic acid (ALA), eicosatrienoic acid (ETE), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), docosapentaenoic acid (DPA); and ω-6 PUFAs: dihomo-γ-linolenic acid (DGLA), linoleic acid (LA), arachidonic acid (AA), docosatetraenoic acid with the common name adrenic acid (AdA).
Results: In the clinically healthy individuals born and permanently living in SAR and AR, there was an age-related increase in LA level combined with an increase in the levels of AA, AdA and DGLA, as well as an increase in ALA level combined with an increase in the levels of EPA, DPA and DHA, the content of which was higher in AR individuals. In all age groups of adult residents of SAR, an increase in the AA/EPA ratio and a decrease in the EPA/DPA and (DGLA EPA)/DHA values were observed relative to similar age groups of AR, which indicates disorders in the PUFA metabolism and intensification of pro-inflammatory eicosanoid synthesis. In AR, a statistically significant increase in both ω-3 and ω-6 PUFAs can be a compensatory-adaptive reaction aimed at preserving the lipid component of cell membranes and reducing the risk of their destruction. An increase in the AA/DGLA ratio can be considered as a hidden risk criterion for the synthesis of pro-inflammatory eicosanoids.

Keywords: 
ω-3 and ω-3 polyunsaturated fatty acids • adults • Arctic and Sub-Arctic regions
References: 
  1. Psota TL, Gebauer SK, Kris-Etherton P. Dietary omega-3 fatty acid intake and cardiovascular risk. Am J Cardiol. 2006;98(4A):3i–18i.
  2. Zhou YE, Kubow S, Dewailly E, Julien P. Decreased activity of desaturase 5 in association with obesity and insulin resistance aggravates declining long-chain n-3 fatty acid status in Cree undergoing dietary transition. Br J Nutr 2009;102(6):888–94. doi: 10.1017/S0007114509301609
  3. Drapkina OM, Shepel' RN. [Omega-3 fatty acids and age-related diseases: realities and prospects]. Rational Pharmacotherapy in Cardiology 2015;11(3):309–316.[Article in Russian].
  4.  Stillwell W, Wassall SR. Docosahexaenoic acid: membrane properties of a unique fatty acid. Chem Phys Lipids. 2003;126(1):1–27.
  5. Novgorodtseva TP, Karaman YK, Zhukova NV, Lobanova EG, Antonyuk MV, Kantur TA. Composition of fatty acids in plasma and erythrocytes and eicosanoids level in patients with metabolic syndrome. Lipids in Health and Disease. 2011; 10(82): 82–86. doi: 10.1186/1476-511X-10-82.
  6. Nazarov PE, Myagkova GI, Grosa NV. [Polyunsaturated fatty acids as universal endogenous bioregulators].  Fine Chemical Technologies. (Vestnik. MITHT). 2009;4(5):3–19.[Article in Russian].
  7. Calder PC. n-3 polyunsaturated fatty acids, inflammation, and inflammatory diseases. Am J Clin Nutr. 2006;83(6 Suppl):1505S–1519S. doi: 10.1093/ajcn/83.6.1505S.
  8.   Lorente-Cebrián S, Costa AG, Navas-Carretero S, Zabala M, Laiglesia LM, Martínez JA, Moreno-Aliaga MJ. An update on the role of omega-3 fatty acids on inflammatory and degenerative diseases. J Physiol Biochem. 2015;71(2):341–9. doi: 10.1007/s13105-015-0395-y.
  9. Novgorodtseva YP, Denisenko YuK, Antonyuk MV, Zhukova NV. [Modification of fatty acid content of cell membranes of erythrocytes at chronic obstructive pulmonary disease]. Bulletin SB RAMS. 2013; 33(5): 64–69.[Article in Russian].
  10. Taratukhin EO. [Atherosclerosis and fatty acids: important association and new therapeutic approach].  Russian Journal of Cardiology. 2011;(5):77–80. [Article in Russian].
  11. Williams JA, Batten SE, Harris M, Rockett BD, Shaikh SR, William S, Wassall SR. Docosahexaenoic and eicosapentaenoic acids segregate differently between raft and nonraft domains. Biophys J. 2012;103(2):228-37. doi: 10.1016/j.bpj.2012.06.016.
  12. Chanchaeva EA. [On the issue of adequate nutrition of the indigenous population of Siberia]. Human Ecology. 2010; 3: 31–34.[Article in Russian].
  13. Innis SM. Omega-3 fatty acid biochemistry: perspectives from human nutrition. Mil Med. 2014;179(11 Suppl):82–7. doi: 10.7205/MILMED-D-14-00147.
  14. Lobanov AA, Bogdanova EN, Andronov SV, Popov AI, Kochkin RA, Kostricin VV, et al. [A study of the traditional diet of residents of the Arctic zone of Western Siberia]. Problems of Nutrition. 2018; 87(5): 31–32. doi: 10.24411/0042-8833-2018-10114.[Article in Russian].
  15. Novgorodtseva TP, Denisenko YK, Antonyuk MV, Yubitskaya NS, Lobanova EG, Zhukova NV. [Blood fatty acids in the development and correction of metabolic syndrome]. Ter Arkh. 2016;88(8):30-34. doi: 10.17116/terarkh201688830-34. [Article in Russian].
  16. Denisenko YuK, Vitkina TI, Kondratyeva EV, Zhukova NV, Nazarenko AV. [Modification of the composition of fatty acids in membranes of mitochondria of platelets in patients with chronic non-obstructive bronchitis]. Health, Medical ecology, Science. 2014;2(56): 59–61. [Article in Russian].
  17. Bichkaev AA, Bichkaeva FA, Volkova NI, Tret’yakova TV, Vlasova OS, Nesterova EV, et al. [The Ratio of Blood Levels of Saturated Fatty Acids and Metabolites of Carbohydrate Metabolism in Residents of the Arctic Aged 22–35 Years]. Journal of Medical and Biological Research. 2017;5(2):44–55. doi: 10.17238/issn2542-1298.2017.5.2.44. [Article in Russian].
  18. Bichkaeva FA. Endocrine regulation of metabolic processes in humans in the North. Ekaterinburg UrB RAS; 2008:303. {In Russian].
  19. Bichkaeva FA. Baranova NF, Vlasova OS, Tret’yakova TV, Nesterova EV, Shengof BA, et al. A method for measuring the mass concentration of fatty acid methyl esters (FAMEs) in blood serum by gas-liquid chromatography. Register of measurement procedures UrB RAS. № 88-16365-001-2019. №  SR.1.31.2019.33742.[In Russian]. 
  20. Denisenko YuK, Novgorodtseva TP, Zhukova NV, Lobanova NG, Antonyuk MV. [Metabolic transformations of polyunsaturated fatty acids in chronic inflammatory diseases of the respiratory system]. Mezdunarodnii zhurnal prikladnih i fundamental'nih issledovanii. 2015;11(2):201–206. [Article in Russian].
  21. Vasilkova TN, Mataev SI. [Metabolic a syndrome in population of the radical people of the extreme North]. Bulletin of the South Ural State University. Series: Education, Health, Physical Culture.  2009;27(160):71–73. [Article in Russian].
  22. Huang CW, Chien YS, Chen YJ, Ajuwon KM, Mersmann HM, Ding ST. Role of n-3 Polyunsaturated Fatty Acids in Ameliorating the Obesity-Induced Metabolic Syndrome in Animal Models and Humans. Int J Mol Sci. 2016;17(10). pii: E1689.
  23. Maki KC, Eren F, Cassens ME, Dicklin MR, Davidson MH. ω-6 Polyunsaturated Fatty Acids and Cardiometabolic Health: Current Evidence, Controversies, and Research Gaps. Adv Nutr. 2018;9(6):688-700. doi: 10.1093/advances/nmy038.
  24. Kromhout D, de Goede J. Update on cardiometabolic health effects of omega-3 fatty acids. Curr Opin Lipidol  2014;25:85–90.
  25. Berger ME, Smesny S, Kim SW, Davey CG, Rice S, Sarnyai Z,et al. Omega-6 to omega-3 polyunsaturated fatty acid ratio and subsequent mood disorders in young people with at-risk mental states: a 7-year longitudinal study. Transl Psychiatry. 2017 Aug 29;7(8):e1220. doi: 10.1038/tp.2017.190.

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Received September 20, 2019.
Accepted October 23, 2019.
©2019 International Medical Research and Development Corporation.