Age-Related Decrease in the Number of Stromal Cell Precursors in Bone Marrow and Spleen May Be Due To both Cellular Pool Depletion and Regulatory Impact of the Body

BASIC RESEARCH
Yulia F. Gorskaya, PhD, ScD*, Tatiana A. Danilova, PhD, ScD, Vladimir G. Nesterenko, PhD, ScD

Gamaleya Institute of Epidemiology and Microbiology, Moscow, Russian Federation

*Corresponding author: Yulia F. Gorskaya, PhD, ScD, Gamaleya Institute of Epidemiology and Microbiology, 18, Gamaleya str., 123098, Moscow, Russian Federation. Tel: 7-499-1935541 E-mail: Uliya.Gorskaya@nearmedic.ru

Abstract: 

The cloning efficiency (CFE-F) of stromal precursor cells (CFU-F) in cultures of heterotopic transplant cells and the quantity of CFU-F in bone marrow transplants from old donors to old recipients (O-O group) has decreased almost eight times, compared with transplants where the bone marrow from young donors has been transplanted to young recipients (Y-Y group). However, in the O-Y group, the CFE-F and the quantity of CFU-F in the transplants increased more than three times compared with the O-O group, however, staying 2.5 times lower than the Y-Y group level. Regarding transplanting bone marrow from young donors to old recipients (Y-O), the CFE-F of transplants decreased approximately two times lower compared with the CFE-F in the Y-Y group. The CFE-F in the cultures of the spleen transplant cells and the amount of CFUF in the transplants of the O-O group have decreased four and six times, respectively, compared with the Y-Y group. However, if the spleen from the old mice was transplanted to young recipients (O-Y), the CFE-F in transplants was noted to increase almost seven times compared with the CFE-F in spleen transplants from old donors to old recipients (O-O), and exceeded the level of the Y-Y group. These data suggest that the age-related decrease in the CFE-F and the quantity of CFUF in the bone marrow are the results of both changes in the stromal tissue (expressed in real reduction in number of CFU-F), and the regulating action of the organism on stromal tissue. Age-related decrease of CFE-F and the amount of CFU-F in spleen could occur, mainly due to the regulating action of the organism.

Keywords: 
stromal cells, age-related changes.
References: 
  1. Bergman RG, Gazit D, Cahn AG, et al. Age-related changes in osteogenic cells in mice. Bone and Mineral 1996; 11:568-577.
  2. Conboy IM, Conboy MJ, Wagers AJ, Girma ER, Weissman IL, et al. Rejuvenation of aged progenitor cells by exposure to a young systemic environment. Nature 2005; 443:760-764.
  3. D'Ippolito G, Schiller PC, Ricordi C, Roos BA, Howard GA. Age-related osteogenic potential of mesenchymal stromal stem cells from human vertebral bone marrow. J Bone Miner Res 1999; 14: 1115-1122.
  4. Chailakhyan RK, Gerasimov IuV, Friedenstein AJ. Bone marrow microenvironment transfer by clones of stromal mechanocytes 1978; 86:705-707.
  5. Friedenstein AJ. Osteogenic stem cells in the bone marrow. Bone and Mineral Res 1999; 7:243-272 .
  6. Friedenstein AJ. Determined and inducible osteogenic precursor cells. Ciba Found Symp 1973; 11: 169-185.
  7. Friedenstein AJ, Chailakhyan RK, Latzinik NV, et al. Stromal cells responsible for transferring the microenvironment of hemopoietic tissues:cloning in vitro and retransplantation in vivo. Transplantation 1974; 17:331-340.
  8. Friedenstein AJ, Gorskaya UF, Shuklina EU, et al. The age-related changes of stromal precursors cells population in hemopoietic and lymphoid organs. Bull Exp Biol Med 1999; 5:550-553.
  9. Friedenstein AJ, Ivanov-Smolenski AA, Chailakhyan RK, et al. Origin of bone marrow stromal mechanocytes in radiochimeras and heterotopic transplants. Experimental hematology 1978; 6:440-444.
  10. Friedenstein AYa, Latzinic NV, Gorskaya YuF, et al. Bone marrow stromal colony formation requires stimulation by haemopoietic cells. Bone and Mineral 1992; 18:199-213.
  11. Gorskaya UF, Kuralesova AI, Shuklina EU,  et al. Enumeration of colony-forming units-fibroblast in bone marrow transplants in mice of different ages. Bull Exp Biol Med 2002; 2:176-179.
  12. Gorskaya UF, Latzinik NV, Shuklina EU, et al. The age-related changes of stromal precursors cells population in hemopoietic and lymphoid organs. Rus J of Immunol 2000; 5:149-155.
  13. Gorskaya UF, Nesterenko VG. Enumeration of colony-forming units-fibroblast in spleen transplants in mice of different ages. Bull Exp Biol Med 2005; 2:196-198.
  14. Kahn JA, Gibbons R, Perkins S, Gazit D. Age related bone loss: a hypothesis and initial assessment in mice. Clin Orthop 1995; 313:69-75.
  15. Kuznetsov SA, Krebsbach PH, Satomura R, et al. Single-colony derived strains of human marrow stromal fibroblasts from bone after transplantation in vivo. J Bone and Mineral Res 1997;12:1335­1347
  16. Kuznetsov SA, Mankani MM, Bianco P, Robey PG. Enumeration of colony-forming units-fibroblast from mouse and human bone marrow in normal and pathological conditions Stem Cell Res 2009; 2:83-94.

Download Article

Int J Biomed. 2011; 1(4):221-224. © 2011 International Medical Research and Development Corporation. All rights reserved.